HPLC purity, chromatographic identity comparison, and quantitative analysis answer separate questions in the current GLUUM. testing program. Each result is tied to the applicable lot and should be interpreted only within the method stated on its Certificate of Analysis.
What HPLC purity measures
High-performance liquid chromatography separates the components of a sample under controlled conditions. The resulting chromatogram reports the relative area of the principal peak and other detected peaks, which supports a method-specific chromatographic purity result.
Standard catalog specification: >=98.0% chromatographic purity unless otherwise identified for a specific compound. CJC-1295 with DAC (92.35%) and IGF-1 LR3 (95.07%) are current catalog exceptions.
How chromatographic identity is assessed
Identity is tested by chromatographic comparison against a reference standard under the applicable method conditions. The identity finding is separate from the HPLC purity percentage: a large principal peak does not, by itself, identify the compound.
What quantitative analysis reports
Quantitative analysis reports the actual measured amount of material in the vial. This is distinct from chromatographic purity, which reports relative detected composition rather than total vial content.
How to read the three results together
| Result | Question answered | Reported evidence |
|---|---|---|
| Identity | Does the sample correspond to the stated compound? | Chromatographic comparison against a reference standard |
| Purity | What share of the detected chromatographic response is the principal component? | HPLC chromatogram and calculated purity |
| Quantitative content | How much material is actually present in the vial? | Quantitative assay result |
These findings should be read separately and matched to the lot code on the applicable COA.
Batch-specific scope
Every batch is independently third-party tested before release for identity, HPLC purity, and quantitative content. A new batch receives a new corresponding COA tied to its lot code. Inventory is held until applicable testing is complete, and material that fails required specifications is not released to fulfillment.
The current independent laboratory is Accumark Labs. Historical or record-specific certificates may retain the exact laboratory named by the issuing record.
SKU-specific additional testing
Sterility and endotoxin testing are not universal catalog claims. Where a qualifying SKU's documentation reports them, endotoxin is tested by USP <85> LAL with a specification of <=5 EU/mL, and sterility uses rapid qPCR screening. Rapid qPCR screening is not USP <71> testing.
Heavy metals, residual solvents, and bioburden are not standard tests and should not be inferred unless a specific record reports them.
What to look for on a COA
- the compound and applicable batch or lot identifier
- HPLC purity with its chromatographic result
- identity reported from comparison against a reference standard
- quantitative content reported as actual measured vial material
- the test date and named analytical source
- any SKU-specific additional panels stated on that report
A full walkthrough is in How to Read a Peptide Certificate of Analysis. Current documents are listed in the COA Library.
Frequently asked questions
Does a purity percentage also prove identity? No. Purity and identity are separate findings. Identity is assessed against a reference standard; purity is calculated from the chromatographic result.
Does HPLC purity report vial content? No. Quantitative analysis separately reports the actual measured material in the vial.
Does every SKU have sterility and endotoxin testing? No. Those panels are SKU-specific and should only be attributed where the applicable documentation reports them.
Does a previous batch's COA apply to a new batch? No. Each new batch has its own corresponding COA tied to the applicable lot code.
Research use only. All compounds referenced by GLUUM. are supplied for laboratory research use only. They are not for human consumption, and nothing in this article is medical, diagnostic, or therapeutic guidance.